Cbs Knockout cell line (Raw 264.7)

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location: Home > Products > KO Cell Lines > Cbs Knockout cell line (Raw 264.7)
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Cbs Knockout cell line (Raw 264.7) Order

Cbs Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0013
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cbs Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Cbs Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Cbs
Gene ID
12411
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript Cbs-211)
Validation: PCR+Sanger Sequencing
Cbs Knockout cell line (Raw 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cbs gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cbs gene knockout Cbs Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Cbs Knockout cell line (Raw 264.7)
Cbs Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0013
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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